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p acor -grna rep -grna scaffold fragments  (GenScript corporation)

 
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    Structured Review

    GenScript corporation p acor -grna rep -grna scaffold fragments
    Plasmid design and construction of the all-in-one plasmid CRISPR-Cas9 system. a Assembly of spacer and donar DNA. The <t>gRNA</t> target under the constitutive promoters for genome editing. The another gRNA rep under the inducible promoter for plasmid curing. b Squence of gRNA rep under the control of P <t>acoR</t> promoter. c Strategy for iterative genome editing in B. subtilis . In the genome editing phase, sgRNA target /Cas9 complex cuts the genome for homologous recombination. In the plasmid curing phase, sgRNA rep /Cas9 complex tragets the repA gene to eliminate the editing plasmid. d Tetracycline sensitivity test for the plasmid self-curing system, 16 clones were picked for tetracycline sensitivity test, and only one single clone showed tetracycline resistance
    P Acor Grna Rep Grna Scaffold Fragments, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+acor+-grna+rep+-grna+scaffold+fragments/pmc09400229-150-12-22?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    p acor -grna rep -grna scaffold fragments - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis"

    Article Title: Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-022-01896-0

    Plasmid design and construction of the all-in-one plasmid CRISPR-Cas9 system. a Assembly of spacer and donar DNA. The gRNA target under the constitutive promoters for genome editing. The another gRNA rep under the inducible promoter for plasmid curing. b Squence of gRNA rep under the control of P acoR promoter. c Strategy for iterative genome editing in B. subtilis . In the genome editing phase, sgRNA target /Cas9 complex cuts the genome for homologous recombination. In the plasmid curing phase, sgRNA rep /Cas9 complex tragets the repA gene to eliminate the editing plasmid. d Tetracycline sensitivity test for the plasmid self-curing system, 16 clones were picked for tetracycline sensitivity test, and only one single clone showed tetracycline resistance
    Figure Legend Snippet: Plasmid design and construction of the all-in-one plasmid CRISPR-Cas9 system. a Assembly of spacer and donar DNA. The gRNA target under the constitutive promoters for genome editing. The another gRNA rep under the inducible promoter for plasmid curing. b Squence of gRNA rep under the control of P acoR promoter. c Strategy for iterative genome editing in B. subtilis . In the genome editing phase, sgRNA target /Cas9 complex cuts the genome for homologous recombination. In the plasmid curing phase, sgRNA rep /Cas9 complex tragets the repA gene to eliminate the editing plasmid. d Tetracycline sensitivity test for the plasmid self-curing system, 16 clones were picked for tetracycline sensitivity test, and only one single clone showed tetracycline resistance

    Techniques Used: Plasmid Preparation, CRISPR, Control, Homologous Recombination, Clone Assay

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Preserving, Plasmid Preparation, Expressing



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